Hotspot

ycf1

SSC · 121072-126384 · 5313 bp

Back to Kummerowia

Pi 0.0191

MarkerSeek score 52.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5313
variable_sites: 101
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACAAGGGGTTTTTGAGCAAA	TTATTTAGGCAGAATACCGC	40.0	40.0	56.2	53.2	234 bp	1.0	42.7
ycf1_p2	ACAAGGGGTTTTTGAGCAAA	ATTATTTAGGCAGAATACCGC	40.0	38.1	56.2	53.8	235 bp	1.0	42.7
ycf1_p3	ACAAGGGGTTTTTGAGCAAAT	TTATTTAGGCAGAATACCGC	38.1	40.0	56.8	53.2	234 bp	1.0	42.7
ycf1_p4	CTTACAAGGGGTTTTTGAGCA	TTATTTAGGCAGAATACCGC	42.9	40.0	56.6	53.2	237 bp	1.0	42.7
ycf1_p5	ACAAGGGGTTTTTGAGCAAATT	TTATTTAGGCAGAATACCGC	36.4	40.0	57.5	53.2	234 bp	1.0	42.7