Hotspot

clpP

LSC · 69412-71475 · 2064 bp

Back to Kummerowia

Pi 0.0147

MarkerSeek score 56.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2064
variable_sites: 30
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	AGGATTCCAAAATGAAATTGCA	TCTTACGTTTCCACACTAAAGT	31.8	36.4	55.1	55.4	2156-2179 bp	1.0	42.0
clpP_p2	AAGGATTCCAAAATGAAATTGCA	TCTTACGTTTCCACACTAAAGT	30.4	36.4	55.9	55.4	2157-2180 bp	1.0	42.0
clpP_p3	AGGATTCCAAAATGAAATTGCAA	TCTTACGTTTCCACACTAAAGT	30.4	36.4	55.9	55.4	2156-2179 bp	1.0	42.0
clpP_p4	AGGATTCCAAAATGAAATTGCA	TTCTTACGTTTCCACACTAAAGT	31.8	34.8	55.1	56.1	2157-2180 bp	1.0	42.0
clpP_p5	AGGATTCCAAAATGAAATTGCAA	TTCTTACGTTTCCACACTAAAGT	30.4	34.8	55.9	56.1	2157-2180 bp	1.0	42.0