Hotspot

rps3-rps19

LSC · 83147-83607 · 461 bp

Back to Kummerowia

Pi 0.051

MarkerSeek score 72.70

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 23
pi: 0.051
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TTGTAGAGGAACCCGACCTT	TAAACTTCCGAGGACATGCG	50.0	50.0	58.0	58.0	1069-1070 bp	1.0	82.8
rps3-rps19_p2	TTTGTAGAGGAACCCGACCT	TAAACTTCCGAGGACATGCG	50.0	50.0	58.0	58.0	1070-1071 bp	1.0	82.8
rps3-rps19_p3	TTGTAGAGGAACCCGACCTT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.0	58.1	1474-1475 bp	1.0	81.8
rps3-rps19_p4	TTGTAGAGGAACCCGACCTT	AAATTTGGGGAAAGCCGGAT	50.0	45.0	58.0	58.0	1578-1579 bp	1.0	81.7
rps3-rps19_p5	TTTGTAGAGGAACCCGACCT	AAATTTGGGGAAAGCCGGAT	50.0	45.0	58.0	58.0	1579-1580 bp	1.0	81.7