Hotspot

psbT-psbN

LSC · 73740-73795 · 56 bp

Back to Kummerowia

Pi 0.0536

MarkerSeek score 77.00

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 3
pi: 0.0536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AACCCCCTAAAGTTCCAACG	CCATTCGAAGAACATGGGGA	50.0	50.0	57.7	57.6	115 bp	1.0	76.5
psbT-psbN_p2	AACCCCCTAAAGTTCCAACG	TCCATTCGAAGAACATGGGG	50.0	50.0	57.7	57.6	116 bp	1.0	76.5
psbT-psbN_p3	AACCCCCTAAAGTTCCAACG	GGCAACCCTCTCAACAACTA	50.0	50.0	57.7	57.4	141 bp	1.0	75.0
psbT-psbN_p4	ACCCCCTAAAGTTCCAACGA	CCATTCGAAGAACATGGGGA	50.0	50.0	58.6	57.6	114 bp	1.0	74.3
psbT-psbN_p5	ACCCCCTAAAGTTCCAACGA	TCCATTCGAAGAACATGGGG	50.0	50.0	58.6	57.6	115 bp	1.0	74.2