Hotspot

trnW-CCA-trnP-UGG

LSC · 65883-66076 · 194 bp

Back to Kummerowia

Pi 0.0278

MarkerSeek score 71.40

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 5
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	GGGTACAAAATGTCACGGGT	50.0	50.0	57.9	58.1	247-261 bp	1.0	84.3
trnW-CCA-trnP-UGG_p2	GCGTTCTACCGAACTGAACT	TGGGTACAAAATGTCACGGG	50.0	50.0	57.9	58.1	248-262 bp	1.0	84.3
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	GTCACGGGTTCAAATCCTGT	50.0	50.0	57.9	58.1	236-250 bp	1.0	84.3
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	CACGGGTTCAAATCCTGTCA	50.0	50.0	57.9	58.1	234-248 bp	1.0	84.3
trnW-CCA-trnP-UGG_p5	GCGTTCTACCGAACTGAACT	TGTCACGGGTTCAAATCCTG	50.0	50.0	57.9	58.1	237-251 bp	1.0	84.3