Hotspot

trnS-GCU-psbI

LSC · 54219-54406 · 188 bp

Back to Kummerowia

Pi 0.0291

MarkerSeek score 60.20

Primer available yes

Label trnS-GCU-psbI

Feature Metadata

label_name: trnS-GCU-psbI
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 5
pi: 0.0291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbI_p1	TGGACTAAAGCGTCGGATTG	CGTAATCCAGGACGTGAAGA	50.0	50.0	58.0	57.1	268-284 bp	1.0	77.9
trnS-GCU-psbI_p2	GTGGACTAAAGCGTCGGATT	CGTAATCCAGGACGTGAAGA	50.0	50.0	58.0	57.1	269-285 bp	1.0	77.9
trnS-GCU-psbI_p3	TGGACTAAAGCGTCGGATTG	CGTAATCCAGGACGTGAAGAA	50.0	47.6	58.0	57.8	268-284 bp	1.0	75.6
trnS-GCU-psbI_p4	GTGGACTAAAGCGTCGGATT	CGTAATCCAGGACGTGAAGAA	50.0	47.6	58.0	57.8	269-285 bp	1.0	75.6
trnS-GCU-psbI_p5	GACTAAAGCGTCGGATTGCT	CGTAATCCAGGACGTGAAGA	50.0	50.0	58.4	57.1	266-282 bp	1.0	75.1