Hotspot

rps2-atpI

LSC · 46988-47311 · 324 bp

Back to Kummerowia

Pi 0.0332

MarkerSeek score 76.10

Primer available yes

Label rps2-atpI

Feature Metadata

label_name: rps2-atpI
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 10
pi: 0.0332
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-atpI_p1	TTGTGAGGGTCGTTCTAGCT	CATGCACTTGGAAACTCCCT	50.0	50.0	58.4	58.1	450-470 bp	1.0	82.1
rps2-atpI_p2	TTTGTGAGGGTCGTTCTAGC	CATGCACTTGGAAACTCCCT	50.0	50.0	57.5	58.1	451-471 bp	1.0	81.4
rps2-atpI_p3	TTGTGAGGGTCGTTCTAGCT	GCCAATAGAAATGTTGCCCG	50.0	50.0	58.4	57.8	426-446 bp	1.0	81.2
rps2-atpI_p4	TGTGAGGGTCGTTCTAGCTA	CATGCACTTGGAAACTCCCT	50.0	50.0	57.5	58.1	449-469 bp	1.0	81.0
rps2-atpI_p5	TTTGTGAGGGTCGTTCTAGC	GCCAATAGAAATGTTGCCCG	50.0	50.0	57.5	57.8	427-447 bp	1.0	80.6