Hotspot

petN-trnC-GCA

LSC · 33877-34357 · 481 bp

Back to Kummerowia

Pi 0.0275

MarkerSeek score 66.70

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 481
variable_sites: 13
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	539-544 bp	1.0	82.5
petN-trnC-GCA_p2	CACTTCTTCCCCATACCACG	TTTCCCCAGTTCAAATCCGG	55.0	50.0	58.0	58.1	587-592 bp	1.0	82.1
petN-trnC-GCA_p3	ACTTCTTCCCCATACCACGA	TTTCCCCAGTTCAAATCCGG	50.0	50.0	58.1	58.1	586-591 bp	1.0	81.9
petN-trnC-GCA_p4	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	57.8	58.1	533-538 bp	1.0	81.1
petN-trnC-GCA_p5	CTTCCCCATACCACGAGAGA	TTTCCCCAGTTCAAATCCGG	55.0	50.0	58.2	58.1	582-587 bp	1.0	80.6