Hotspot

trnS-UGA-psbC

LSC · 27385-27611 · 227 bp

Back to Kummerowia

Pi 0.0705

MarkerSeek score 78.00

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 16
pi: 0.0705
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	TCATTTATGGCACGCAGGAA	55.0	45.0	58.0	57.9	422 bp	1.0	81.9
trnS-UGA-psbC_p2	ATAGCCCCGGTCTTGAAAAC	TCATTTATGGCACGCAGGAA	50.0	45.0	57.9	57.9	402 bp	1.0	81.2
trnS-UGA-psbC_p3	GCTTTTGGAGAGATGGCTGA	TCATTTATGGCACGCAGGAA	50.0	45.0	57.9	57.9	429 bp	1.0	81.1
trnS-UGA-psbC_p4	AGCTTTTGGAGAGATGGCTG	TCATTTATGGCACGCAGGAA	50.0	45.0	57.9	57.9	430 bp	1.0	81.0
trnS-UGA-psbC_p5	GAGAGATGGCTGAGTGGTTG	TTTATGGCACGCAGGAAGAG	55.0	50.0	58.0	58.3	419 bp	1.0	80.8