Hotspot

psbZ-trnS-UGA

LSC · 26963-27291 · 329 bp

Back to Kummerowia

Pi 0.0214

MarkerSeek score 61.70

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 329
variable_sites: 7
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	ACTTGACCAACCTTCAGGAG	CAACCACTCAGCCATCTCTC	50.0	55.0	57.1	58.0	455-485 bp	1.0	73.8
psbZ-trnS-UGA_p2	ACTTGACCAACCTTCAGGAG	TTCGCCGAGAAAAAGGAGAG	50.0	50.0	57.1	57.9	540-570 bp	1.0	73.1
psbZ-trnS-UGA_p3	ACTTGACCAACCTTCAGGAG	GTTTTCAAGACCGGGGCTAT	50.0	50.0	57.1	57.9	475-505 bp	1.0	73.0
psbZ-trnS-UGA_p4	GGAGAAGCAAATACAACGGG	CAACCACTCAGCCATCTCTC	50.0	55.0	56.8	58.0	439-469 bp	1.0	71.8
psbZ-trnS-UGA_p5	CTTGACCAACCTTCAGGAGA	CAACCACTCAGCCATCTCTC	50.0	55.0	56.8	58.0	454-484 bp	1.0	71.5