Hotspot

ycf3-psaA

LSC · 20132-20808 · 677 bp

Back to Kummerowia

Pi 0.0262

MarkerSeek score 71.40

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 17
pi: 0.0262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	TTGGTTGAAGATCACTGGGC	TTTCACTTCTGGTTCCGGTG	50.0	50.0	58.1	58.0	741 bp	1.0	83.9
ycf3-psaA_p2	TTGGTTGAAGATCACTGGGC	AATGACCTGGTTTTGCCCAT	50.0	45.0	58.1	58.0	802 bp	1.0	83.6
ycf3-psaA_p3	TTGGTTGAAGATCACTGGGC	ATGACCTGGTTTTGCCCATT	50.0	45.0	58.1	58.0	801 bp	1.0	83.6
ycf3-psaA_p4	TTGGTTGAAGATCACTGGGC	TGGTTTTGCCCATTCCTCAA	50.0	45.0	58.1	57.9	795 bp	1.0	83.2
ycf3-psaA_p5	TGGTTTAATCAAGCCGCTGA	ATGACCTGGTTTTGCCCATT	45.0	45.0	57.8	58.0	875 bp	1.0	82.4