Hotspot

trnL-UAA-trnT-UGU

LSC · 15705-16624 · 920 bp

Back to Kummerowia

Pi 0.0197

MarkerSeek score 65.70

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 920
variable_sites: 17
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	GCGTCTACCTATTTCGCCAT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	918-976 bp	1.0	83.2
trnL-UAA-trnT-UGU_p2	GCGTCTACCTATTTCGCCAT	ATCGAGAAAAGCCGGCTATC	50.0	50.0	57.8	57.8	970-1028 bp	1.0	82.9
trnL-UAA-trnT-UGU_p3	GCGTCTACCTATTTCGCCAT	GGATTCGAACCGATGACCAT	50.0	50.0	57.8	57.8	950-1008 bp	1.0	82.3
trnL-UAA-trnT-UGU_p4	GCGTCTACCTATTTCGCCAT	AAATCGAGAAAAGCCGGCTA	50.0	45.0	57.8	57.6	972-1030 bp	1.0	81.0
trnL-UAA-trnT-UGU_p5	GCGTCTACCTATTTCGCCAT	CTAACCTCTGAGCTAAGCGG	50.0	55.0	57.8	57.5	910-968 bp	1.0	80.2