Hotspot

trnK-UUU-rbcL

LSC · 5919-6764 · 846 bp

Back to Kummerowia

Pi 0.0221

MarkerSeek score 61.30

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 18
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGAGTCGCTCTTAAAAGCCG	GAGCCGTATTTGCCAGAGAA	50.0	50.0	58.0	58.0	1555-1564 bp	1.0	82.7
trnK-UUU-rbcL_p2	AGTCGCTCTTAAAAGCCGAG	GAGCCGTATTTGCCAGAGAA	50.0	50.0	58.0	58.0	1553-1562 bp	1.0	82.7
trnK-UUU-rbcL_p3	GAGTCGCTCTTAAAAGCCGA	GAGCCGTATTTGCCAGAGAA	50.0	50.0	58.0	58.0	1554-1563 bp	1.0	82.6
trnK-UUU-rbcL_p4	AGTCGCTCTTAAAAGCCGAG	AAGAGCCGTATTTGCCAGAG	50.0	50.0	58.0	58.0	1555-1564 bp	1.0	82.6
trnK-UUU-rbcL_p5	AAACCTATCAACGGACTCGC	GAGCCGTATTTGCCAGAGAA	50.0	50.0	58.0	58.0	2140-2149 bp	1.0	82.1