Hotspot

ycf92

Genome · 54433-55257 · 825 bp

Back to Kumanoa

Pi 0.214

MarkerSeek score 80.20

Primer available yes

Label ycf92

Feature Metadata

label_name: ycf92
feature_type: gene
region: Genome
length_bp: 825
variable_sites: 174
pi: 0.214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf92_p1	GCGAGTATTCGGTGGAATGT	TTTGATCGAAAGCGCGAGTA	50.0	45.0	58.1	58.0	2112 bp	0.5	59.4
ycf92_p2	GCGAGTATTCGGTGGAATGT	TGCGTATACCAATTTCGCCA	50.0	45.0	58.1	58.0	1745 bp	0.5	59.3
ycf92_p3	GCAGAAAGATCCGTCGCTAA	TTTGATCGAAAGCGCGAGTA	50.0	45.0	58.1	58.0	2091 bp	0.5	59.3
ycf92_p4	GCAGAAAGATCCGTCGCTAA	TGCGTATACCAATTTCGCCA	50.0	45.0	58.1	58.0	1724 bp	0.5	59.2
ycf92_p5	GCGAGTATTCGGTGGAATGT	AAAGCGCGAGTAGTAGAAGC	50.0	50.0	58.1	58.1	2104 bp	0.5	58.8