Hotspot

ycf80

Genome · 70882-72378 · 1497 bp

Back to Kumanoa

Pi 0.169

MarkerSeek score 79.80

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1497
variable_sites: 251
pi: 0.169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	TGCCTGGCTTAAGCAGAAAA	TGTTTTGAGCGTCAGATGCT	45.0	45.0	58.0	58.1	2564 bp	0.5	59.0
ycf80_p2	TTGCCTGGCTTAAGCAGAAA	TGTTTTGAGCGTCAGATGCT	45.0	45.0	58.0	58.1	2565 bp	0.5	59.0
ycf80_p3	TTTGCCTGGCTTAAGCAGAA	TGTTTTGAGCGTCAGATGCT	45.0	45.0	58.0	58.1	2566 bp	0.5	59.0
ycf80_p4	AAAACAAGCTCCTCCTGGTG	TGTTTTGAGCGTCAGATGCT	50.0	45.0	58.0	58.1	2536 bp	0.5	59.0
ycf80_p5	CCCGCTGCTAGACAATTGAT	TGTTTTGAGCGTCAGATGCT	50.0	45.0	58.0	58.1	2301 bp	0.5	58.7