Hotspot

upp

Genome · 136294-136920 · 627 bp

Back to Kumanoa

Pi 0.192

MarkerSeek score 79.30

Primer available yes

Label upp

Feature Metadata

label_name: upp
feature_type: gene
region: Genome
length_bp: 627
variable_sites: 120
pi: 0.192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
upp_p1	TTGGTGGCGAAACAATGAGA	GTGAGCCTATTGAGCGAACA	45.0	50.0	58.0	58.0	1360 bp	0.5	59.7
upp_p2	TTAATGCGATCCCCAAGTGG	GTGAGCCTATTGAGCGAACA	50.0	50.0	57.9	58.0	1391 bp	0.5	59.5
upp_p3	GCATCTGCTCAAGGACCTAC	GTGAGCCTATTGAGCGAACA	55.0	50.0	58.1	58.0	1427 bp	0.5	59.5
upp_p4	CTGCTGCCGGTTTTGAAAAA	GTGAGCCTATTGAGCGAACA	45.0	50.0	58.1	58.0	1060 bp	0.5	59.4
upp_p5	TGCTGCCGGTTTTGAAAAAG	GTGAGCCTATTGAGCGAACA	45.0	50.0	58.1	58.0	1059 bp	0.5	59.4