Hotspot

rps2

Genome · 46013-46714 · 702 bp

Back to Kumanoa

Pi 0.103

MarkerSeek score 80.20

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: Genome
length_bp: 702
variable_sites: 72
pi: 0.103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TTGCAAGCAAACTGGATTGG	CTGTTGTGCTACGATGAGCT	45.0	50.0	57.5	58.0	1296 bp	0.5	55.7
rps2_p2	TTGCAAGCAAACTGGATTGG	TCTGTTGTGCTACGATGAGC	45.0	50.0	57.5	58.0	1297 bp	0.5	55.7
rps2_p3	TTGCAAGCAAACTGGATTGG	ACAATCCATCATACCCGCAC	45.0	50.0	57.5	58.0	1190 bp	0.5	55.5
rps2_p4	TTGCAAGCAAACTGGATTGG	TGCAGCTATTTGCATGGCTA	45.0	45.0	57.5	57.9	1433 bp	0.5	55.3
rps2_p5	TTGCAAGCAAACTGGATTGG	CCCGCACCAGTTTTAACTCT	45.0	50.0	57.5	58.1	1177 bp	0.5	54.9