Hotspot

chlB

Genome · 12302-15826 · 3525 bp

Back to Kumanoa

Pi 0.134

MarkerSeek score 75.10

Primer available yes

Label chlB

Feature Metadata

label_name: chlB
feature_type: gene
region: Genome
length_bp: 3525
variable_sites: 470
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB_p1	AGAACCTCAATGGACAGCAA	GGAGTATGAAACAACCAGTTGG	45.0	45.5	57.0	57.2	951 bp	0.5	30.2
chlB_p2	ACCTCAATGGACAGCAAAACT	GGAGTATGAAACAACCAGTTGG	42.9	45.5	58.1	57.2	948 bp	0.5	29.5
chlB_p3	AGAACCTCAATGGACAGCAAA	GGAGTATGAAACAACCAGTTGG	42.9	45.5	57.8	57.2	951 bp	0.5	28.0
chlB_p4	AGAACCTCAATGGACAGCAA	ACAAGGAGTATGAAACAACCAGT	45.0	39.1	57.0	58.0	955 bp	0.5	28.0
chlB_p5	ACCTCAATGGACAGCAAAACT	ACAAGGAGTATGAAACAACCAGT	42.9	39.1	58.1	58.0	952 bp	0.5	27.2