Hotspot

clpC-rpl19

Genome · 157176-157841 · 666 bp

Back to Kumanoa

Pi 0.192

MarkerSeek score 79.30

Primer available yes

Label clpC-rpl19

Feature Metadata

label_name: clpC-rpl19
feature_type: igs
region: Genome
length_bp: 666
variable_sites: 128
pi: 0.192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC-rpl19_p1	CCCAAACGTCTAGCTTCCTC	TACATTGTAAATGGGCCGGG	55.0	50.0	58.0	57.9	1453 bp	0.5	59.4
clpC-rpl19_p2	CCCAAACGTCTAGCTTCCTC	GACTCGAACCAGTGACTTCC	55.0	55.0	58.0	57.9	1244 bp	0.5	59.4
clpC-rpl19_p3	CCCAAACGTCTAGCTTCCTC	GGACTCGAACCAGTGACTTC	55.0	55.0	58.0	57.9	1245 bp	0.5	59.4
clpC-rpl19_p4	GTTGGTGTTTTGCTACGAGC	TACATTGTAAATGGGCCGGG	50.0	50.0	58.0	57.9	1873 bp	0.5	59.3
clpC-rpl19_p5	TTATGCCCCAAACGTCTAGC	TACATTGTAAATGGGCCGGG	50.0	50.0	58.0	57.9	1459 bp	0.5	59.3