Hotspot

BIZ62_gt019-BIZ62_gr001

Genome · 130643-130695 · 53 bp

Back to Kumanoa

Pi 0.14

MarkerSeek score 81.60

Primer available yes

Label BIZ62_gt019-BIZ62_gr001

Feature Metadata

label_name: BIZ62_gt019-BIZ62_gr001
feature_type: igs
region: Genome
length_bp: 53
variable_sites: 7
pi: 0.14
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
BIZ62_gt019-BIZ62_gr001_p1	AACCATGGACCTCACCCTTA	GACTGGGGTGAAGTCGTAAC	50.0	55.0	57.9	57.9	163-165 bp	1.0	83.8
BIZ62_gt019-BIZ62_gr001_p2	TGGACCTCACCCTTATCAGG	GACTGGGGTGAAGTCGTAAC	55.0	55.0	58.1	57.9	158-160 bp	1.0	83.5
BIZ62_gt019-BIZ62_gr001_p3	AACCATGGACCTCACCCTTA	GGGGTGAAGTCGTAACAAGG	50.0	55.0	57.9	58.2	159-161 bp	1.0	83.0
BIZ62_gt019-BIZ62_gr001_p4	AACCATGGACCTCACCCTTA	ACGCCTGAAACCGTTACTTT	50.0	45.0	57.9	58.0	220-222 bp	1.0	82.9
BIZ62_gt019-BIZ62_gr001_p5	TGGACCTCACCCTTATCAGG	ACGCCTGAAACCGTTACTTT	55.0	45.0	58.1	58.0	215-217 bp	1.0	82.4