Hotspot

rpl34-BIZ62_gt015

Genome · 105930-106420 · 491 bp

Back to Kumanoa

Pi 0.193
MarkerSeek score 81.20
Primer available yes
Label rpl34-BIZ62_gt015

Feature Metadata

label_name
rpl34-BIZ62_gt015
feature_type
igs
region
Genome
length_bp
491
variable_sites
92
pi
0.193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl34-BIZ62_gt015_p1 CCTTGCAAAGTTCTTTTTGTCA GTTCAAATCCTGCTATCCCGA 36.4 47.6 56.2 57.8 535-537 bp 1.0 50.3
rpl34-BIZ62_gt015_p2 CCTTGCAAAGTTCTTTTTGTCA AGGTTCAAATCCTGCTATCCC 36.4 47.6 56.2 57.4 537-539 bp 1.0 50.2
rpl34-BIZ62_gt015_p3 CCTTGCAAAGTTCTTTTTGTCA TTCAAATCCTGCTATCCCGA 36.4 45.0 56.2 56.3 534-536 bp 1.0 50.2
rpl34-BIZ62_gt015_p4 CCTTGCAAAGTTCTTTTTGTCAT GTTCAAATCCTGCTATCCCGA 34.8 47.6 56.7 57.8 535-537 bp 1.0 50.2
rpl34-BIZ62_gt015_p5 CCTTGCAAAGTTCTTTTTGTCA GTTCAAATCCTGCTATCCCG 36.4 50.0 56.2 56.3 535-537 bp 1.0 50.2