Hotspot

BIZ62_gt013-BIZ62_gt014

Genome · 101142-101192 · 51 bp

Back to Kumanoa

Pi 0.125
MarkerSeek score 82.60
Primer available yes
Label BIZ62_gt013-BIZ62_gt014

Feature Metadata

label_name
BIZ62_gt013-BIZ62_gt014
feature_type
igs
region
Genome
length_bp
51
variable_sites
6
pi
0.125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
BIZ62_gt013-BIZ62_gt014_p1 CAGGTGGTCACTGGTTCAAA GGTCCCTGTCCTAATCCCTT 50.0 55.0 57.9 58.1 115-118 bp 1.0 84.9
BIZ62_gt013-BIZ62_gt014_p2 CAGGTGGTCACTGGTTCAAA AAGGTCCCTGTCCTAATCCC 50.0 55.0 57.9 58.1 117-120 bp 1.0 84.8
BIZ62_gt013-BIZ62_gt014_p3 CAGGTGGTCACTGGTTCAAA ATCCCTTAGACGATGGACCC 50.0 55.0 57.9 58.3 102-105 bp 1.0 84.3
BIZ62_gt013-BIZ62_gt014_p4 TTACAAGCAGGTGGTCACTG GGTCCCTGTCCTAATCCCTT 50.0 55.0 57.7 58.1 122-125 bp 1.0 83.0
BIZ62_gt013-BIZ62_gt014_p5 TTACAAGCAGGTGGTCACTG AAGGTCCCTGTCCTAATCCC 50.0 55.0 57.7 58.1 124-127 bp 1.0 82.9