Hotspot

ycf80-BIZ62_gs002

Genome · 72379-72659 · 281 bp

Back to Kumanoa

Pi 0.211

MarkerSeek score 74.60

Primer available yes

Label ycf80-BIZ62_gs002

Feature Metadata

label_name: ycf80-BIZ62_gs002
feature_type: igs
region: Genome
length_bp: 281
variable_sites: 59
pi: 0.211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80-BIZ62_gs002_p1	TGGGAATCGATACGACCAGA	TGTTTTGAGCGTCAGATGCT	50.0	45.0	57.9	58.1	924 bp	0.5	58.6
ycf80-BIZ62_gs002_p2	TGGGAATCGATACGACCAGA	TTCAAGTCCTTCCTTGCTCG	50.0	50.0	57.9	57.8	1190 bp	0.5	58.1
ycf80-BIZ62_gs002_p3	TGGGAATCGATACGACCAGA	TTAACATGGACGTGGGTTCG	50.0	50.0	57.9	58.2	754 bp	0.5	57.9
ycf80-BIZ62_gs002_p4	TGGGAATCGATACGACCAGA	ATAGAGTGCCAGATTCCGGT	50.0	50.0	57.9	58.2	1225 bp	0.5	57.9
ycf80-BIZ62_gs002_p5	TACGCGTTAGAAGCTCTTCG	TGTTTTGAGCGTCAGATGCT	50.0	45.0	57.8	58.1	1178 bp	0.5	57.7