Hotspot

petP-ycf45

Genome · 8989-9307 · 319 bp

Back to Kumanoa

Pi 0.207

MarkerSeek score 73.50

Primer available yes

Label petP-ycf45

Feature Metadata

label_name: petP-ycf45
feature_type: igs
region: Genome
length_bp: 319
variable_sites: 66
pi: 0.207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petP-ycf45_p1	AGATTCGTTTGGTGCAGGAG	TTGTTTTCCCTACACCAGGC	50.0	50.0	58.2	58.0	1321 bp	0.5	58.4
petP-ycf45_p2	GATTCGTTTGGTGCAGGAGA	TTGTTTTCCCTACACCAGGC	50.0	50.0	58.2	58.0	1320 bp	0.5	58.4
petP-ycf45_p3	AAGATTCGTTTGGTGCAGGA	TTGTTTTCCCTACACCAGGC	45.0	50.0	57.7	58.0	1322 bp	0.5	57.8
petP-ycf45_p4	ATTCGTTTGGTGCAGGAGAA	TTGTTTTCCCTACACCAGGC	45.0	50.0	57.7	58.0	1319 bp	0.5	57.8
petP-ycf45_p5	AGATTCGTTTGGTGCAGGAG	AGGACGCCTACCTAAATCCA	50.0	50.0	58.2	57.8	1035 bp	0.5	57.1