Hotspot

trnV-GAC-rps12

IRa · 128254-129278 · 1025 bp

Back to Kuepferia

Pi 0.0359

MarkerSeek score 67.40

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 1025
variable_sites: 49
pi: 0.0359
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	CCTCAGCCTACGGGGTATTA	TACTCCGACAGCATCTAGGG	55.0	55.0	58.0	58.0	1539-1704 bp	1.0	86.3
trnV-GAC-rps12_p2	CCTCAGCCTACGGGGTATTA	TCCAGTCCCCTTACGAAACT	55.0	50.0	58.0	58.0	1679-1844 bp	1.0	86.1
trnV-GAC-rps12_p3	CCTCAGCCTACGGGGTATTA	AGCGATTCACATGGCATCAT	55.0	45.0	58.0	58.0	1622-1787 bp	1.0	86.1
trnV-GAC-rps12_p4	CATTGCGGAAAATTCCCCAC	TACTCCGACAGCATCTAGGG	50.0	55.0	58.0	58.0	1713-1878 bp	1.0	86.0
trnV-GAC-rps12_p5	CATTGCGGAAAATTCCCCAC	TCCAGTCCCCTTACGAAACT	50.0	50.0	58.0	58.0	1853-2018 bp	1.0	85.8