Hotspot

ndhF-rpl32

SSC · 104485-105145 · 661 bp

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Pi 0.0318
MarkerSeek score 69.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
661
variable_sites
29
pi
0.0318
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCACCCATATTGATATATCTGTTGC AGCGGTCCAATATCCCTTCC 40.0 55.0 57.7 59.2 694-814 bp 1.0 47.1
ndhF-rpl32_p2 CCACCCATATTGATATATCTGTTGC GCGGTCCAATATCCCTTCCT 40.0 55.0 57.7 59.2 693-813 bp 1.0 47.1
ndhF-rpl32_p3 CCACCCATATTGATATATCTGTTGC AAGCGGTCCAATATCCCTTC 40.0 50.0 57.7 57.4 695-815 bp 1.0 47.0
ndhF-rpl32_p4 CCACCCATATTGATATATCTGTTGC AAAGCGGTCCAATATCCCTTC 40.0 47.6 57.7 58.1 696-816 bp 1.0 47.0
ndhF-rpl32_p5 CCACCCATATTGATATATCTGTTGC AAAGCGGTCCAATATCCCTT 40.0 45.0 57.7 56.9 696-816 bp 1.0 47.0