Hotspot

trnR-ACG-trnN-GUU

IRb · 100849-101124 · 276 bp

Back to Kuepferia

Pi 0.309

MarkerSeek score 79.50

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 276
variable_sites: 106
pi: 0.309
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	296-474 bp	1.0	89.0
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	300-478 bp	1.0	88.1
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	GGTTCGAATCCTACTTGGGG	55.0	55.0	57.8	57.7	288-466 bp	1.0	87.7
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TTGGTCGTAGGTTCGAATCC	55.0	50.0	57.8	57.3	297-475 bp	1.0	85.0
trnR-ACG-trnN-GUU_p5	GGCCTGTAGCTCAGAGGATT	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.9	58.2	307-485 bp	1.0	83.8