Hotspot

rps12-trnV-GAC

IRb · 92138-93162 · 1025 bp

Back to Kuepferia

Pi 0.0352
MarkerSeek score 67.00
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1025
variable_sites
48
pi
0.0352
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TACTCCGACAGCATCTAGGG CCTCAGCCTACGGGGTATTA 55.0 55.0 58.0 58.0 1539-1704 bp 1.0 86.2
rps12-trnV-GAC_p2 AGCGATTCACATGGCATCAT CCTCAGCCTACGGGGTATTA 45.0 55.0 58.0 58.0 1622-1787 bp 1.0 86.1
rps12-trnV-GAC_p3 TCCAGTCCCCTTACGAAACT CCTCAGCCTACGGGGTATTA 50.0 55.0 58.0 58.0 1679-1844 bp 1.0 86.0
rps12-trnV-GAC_p4 TACTCCGACAGCATCTAGGG CATTGCGGAAAATTCCCCAC 55.0 50.0 58.0 58.0 1713-1878 bp 1.0 86.0
rps12-trnV-GAC_p5 TCCAGTCCCCTTACGAAACT CATTGCGGAAAATTCCCCAC 50.0 50.0 58.0 58.0 1853-2018 bp 1.0 85.8