Hotspot

rpl14-rpl16

LSC · 75918-76018 · 101 bp

Back to Kuepferia

Pi 0.0594

MarkerSeek score 72.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 101
variable_sites: 9
pi: 0.0594
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	CGATTGCTAGCGCCTATGAT	GCGATTTCAATAGCAGCGTC	50.0	50.0	58.0	58.0	250-259 bp	1.0	83.7
rpl14-rpl16_p2	CGATTGCTAGCGCCTATGAT	CGATGGGAACGATGGAAACT	50.0	50.0	58.0	58.0	857-872 bp	1.0	83.3
rpl14-rpl16_p3	CGCGCTGTTTTCTCATGTTT	GCGATTTCAATAGCAGCGTC	45.0	50.0	58.0	58.0	814-835 bp	1.0	83.1
rpl14-rpl16_p4	CGATTGCTAGCGCCTATGAT	GGAACGAAGCCCAGATGAAT	50.0	50.0	58.0	58.0	785-794 bp	1.0	82.1
rpl14-rpl16_p5	CGATTGCTAGCGCCTATGAT	CGCCCGCGAAAACTTTATTT	50.0	45.0	58.0	58.0	707 bp	0.333333	53.1