Hotspot

petB-petD

LSC · 71403-71601 · 199 bp

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Pi 0.0842

MarkerSeek score 76.10

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 25
pi: 0.0842
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	TATTGCCCCTTCTTACTGCC	TTCAAGTCACCCACTCCCAT	50.0	50.0	57.3	58.6	299-306 bp	1.0	76.9
petB-petD_p2	CCCCTTCTTACTGCCGTATT	TTCAAGTCACCCACTCCCAT	50.0	50.0	57.0	58.6	294-301 bp	1.0	74.8
petB-petD_p3	TGTATTGCCCCTTCTTACTGC	TTCAAGTCACCCACTCCCAT	47.6	50.0	58.0	58.6	301-308 bp	1.0	74.7
petB-petD_p4	ACACACTTTTGTATTGCCCCT	TTCAAGTCACCCACTCCCAT	42.9	50.0	58.0	58.6	310-317 bp	1.0	74.1
petB-petD_p5	TATTGCCCCTTCTTACTGCC	TTCAAGTCACCCACTCCCA	50.0	52.6	57.3	58.1	299-306 bp	1.0	72.8