Hotspot

ndhC-trnV-UAC

LSC · 47426-47733 · 308 bp

Back to Kuepferia

Pi 0.202

MarkerSeek score 74.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 308
variable_sites: 83
pi: 0.202
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.9	57.9	375-428 bp	1.0	90.3
ndhC-trnV-UAC_p2	AATTGGGGCTAAAACTCCGG	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.2	57.9	379-432 bp	1.0	90.2
ndhC-trnV-UAC_p3	TCTCACTAAAGAAGGGGCCA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.0	57.9	967-1014 bp	0.666667	73.9
ndhC-trnV-UAC_p4	TCTCACTAAAGAAGGGGCCA	GGTCTGATTGTAGTGCCGTT	50.0	50.0	58.0	57.9	1451-1497 bp	0.666667	72.6
ndhC-trnV-UAC_p5	TCTCACTAAAGAAGGGGCCA	AGGGCTATAGCTCAGTTGGT	50.0	50.0	58.0	57.8	1615-1655 bp	0.666667	71.6