Hotspot

trnG-GCC-trnfM-CAU

LSC · 33751-33883 · 133 bp

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Pi 0.0602
MarkerSeek score 72.80
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
133
variable_sites
10
pi
0.0602
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 GTTCGATTCCCGCTATCCG CTTGAGGTCACGGGTTCAAA 57.9 50.0 58.2 58.0 162-252 bp 1.0 78.6
trnG-GCC-trnfM-CAU_p2 GGTTCGATTCCCGCTATCC CTTGAGGTCACGGGTTCAAA 57.9 50.0 57.8 58.0 163-253 bp 1.0 78.4
trnG-GCC-trnfM-CAU_p3 GTTCGATTCCCGCTATCCG GGGTAGAGCAGTTTGGTAGC 57.9 55.0 58.2 58.0 198-288 bp 1.0 78.0
trnG-GCC-trnfM-CAU_p4 GGGTTCGATTCCCGCTATC GGGTAGAGCAGTTTGGTAGC 57.9 55.0 57.8 58.0 200-290 bp 1.0 77.8
trnG-GCC-trnfM-CAU_p5 GGTTCGATTCCCGCTATCC GGGTAGAGCAGTTTGGTAGC 57.9 55.0 57.8 58.0 199-289 bp 1.0 77.8