Hotspot

rpoB-trnC-GCA

LSC · 24935-25796 · 862 bp

Back to Kuepferia

Pi 0.0391

MarkerSeek score 74.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 862
variable_sites: 48
pi: 0.0391
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	TTTTATCAGCCGACGAAGGG	50.0	50.0	58.0	58.0	1483-1744 bp	1.0	86.4
rpoB-trnC-GCA_p2	CCGCCTACACAAGCAAATTG	TTTTATCAGCCGACGAAGGG	50.0	50.0	58.0	58.0	1675-1936 bp	1.0	86.3
rpoB-trnC-GCA_p3	CCGCCTACACAAGCAAATTG	CATTTCCTACCAGGCTGTCC	50.0	55.0	58.0	58.0	1891-2161 bp	0.666667	69.6
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	CCATTTCCTACCAGGCTGTC	50.0	55.0	58.0	58.0	1892-2162 bp	0.666667	69.6
rpoB-trnC-GCA_p5	TAGACACTCCCTCATTGCCA	TTTTATCAGCCGACGAAGGG	50.0	50.0	58.0	58.0	1002 bp	0.333333	52.8