Hotspot

atpH-atpI

LSC · 11885-12750 · 866 bp

Back to Kuepferia

Pi 0.048

MarkerSeek score 82.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 866
variable_sites: 58
pi: 0.048
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AAGCAAGTCCTACAGCCAAC	TAGGTGAATCCATGGAAGGC	50.0	50.0	58.1	57.0	821-985 bp	1.0	80.0
atpH-atpI_p2	CAGCAGCAATAACAGAAGCG	TAGGTGAATCCATGGAAGGC	50.0	50.0	57.8	57.0	800-964 bp	1.0	79.6
atpH-atpI_p3	GAAGCAAGTCCTACAGCCAA	TAGGTGAATCCATGGAAGGC	50.0	50.0	57.8	57.0	822-986 bp	1.0	79.3
atpH-atpI_p4	AAGCAAGTCCTACAGCCAAC	GGTGAATCCATGGAAGGCC	50.0	57.9	58.1	58.2	819-983 bp	1.0	78.7
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	GGTGAATCCATGGAAGGCC	50.0	57.9	57.8	58.2	798-962 bp	1.0	78.3