Hotspot

ycf2

IRb · 87751-92298 · 4548 bp

Back to Krascheninnikovia

Pi 0.0011
MarkerSeek score 53.80
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
IRb
length_bp
4548
variable_sites
5
pi
0.0011
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 CAGAACCGAATTCCTTCTGGT CGTTAAAGTGAAGAACTGAACCA 47.6 39.1 57.9 57.3 269 bp 1.0 42.6
ycf2_p2 CAGAACCGAATTCCTTCTGGT AGTGAAGAACTGAACCAAGA 47.6 40.0 57.9 54.1 263 bp 1.0 41.5
ycf2_p3 CAGAACCGAATTCCTTCTGGT TCTCTTTCTTCCTCATCAATCGA 47.6 39.1 57.9 57.1 241 bp 1.0 41.1
ycf2_p4 CAGAACCGAATTCCTTCTGGT TCGTTAAAGTGAAGAACTGAACC 47.6 39.1 57.9 57.0 270 bp 1.0 40.9
ycf2_p5 CAGAACCGAATTCCTTCTGGT AGTGAAGAACTGAACCAAGAA 47.6 38.1 57.9 55.0 263 bp 1.0 40.9