Hotspot

rps19

LSC · 84625-84903 · 279 bp

Back to Krascheninnikovia

Pi 0.00717

MarkerSeek score 43.90

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 2
pi: 0.00717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TCGTGCTTTGTCAACAGACA	TGGGTAGAGCCGGATCTAAG	45.0	55.0	58.0	58.0	776 bp	1.0	80.2
rps19_p2	TCGTGCTTTGTCAACAGACA	ATACACTATGGGGATGGCGA	45.0	50.0	58.0	58.0	1110 bp	1.0	80.0
rps19_p3	TCGTGCTTTGTCAACAGACA	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.0	58.1	677 bp	1.0	79.9
rps19_p4	CCACACAATAATTCGCGTCG	TGGGTAGAGCCGGATCTAAG	50.0	55.0	57.9	58.0	1159 bp	1.0	79.7
rps19_p5	CGACCACACAATAATTCGCG	TGGGTAGAGCCGGATCTAAG	50.0	55.0	57.9	58.0	1162 bp	1.0	79.7