Hotspot

trnN-GUU-ycf1

IRb · 107302-107829 · 528 bp

Back to Krascheninnikovia

Pi 0.00415

MarkerSeek score 63.40

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 528
variable_sites: 2
pi: 0.00415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	AGGATTCGAACCTACGACCA	ATAGAGTCCGACCACAACGA	50.0	50.0	58.2	58.2	628-674 bp	1.0	81.6
trnN-GUU-ycf1_p2	AATCAGTTAACAGCCGACCG	ATAGAGTCCGACCACAACGA	50.0	50.0	58.3	58.2	609-655 bp	1.0	80.7
trnN-GUU-ycf1_p3	CCCCAAGTAGGATTCGAACC	ATAGAGTCCGACCACAACGA	55.0	50.0	57.7	58.2	636-682 bp	1.0	80.3
trnN-GUU-ycf1_p4	CGAGCCCCTTCATTCTGAAT	ATAGAGTCCGACCACAACGA	50.0	50.0	57.7	58.2	682-728 bp	1.0	80.2
trnN-GUU-ycf1_p5	AGGATTCGAACCTACGACCA	AATAGAGTCCGACCACAACG	50.0	50.0	58.2	57.4	629-675 bp	1.0	77.8