Hotspot

psbE-petL

LSC · 65004-66214 · 1211 bp

Back to Krascheninnikovia

Pi 0.00165
MarkerSeek score 59.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1211
variable_sites
2
pi
0.00165
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA AGCGGAGGTTATAGTTAAAGCT 50.0 40.9 57.9 57.0 1375-1377 bp 1.0 55.5
psbE-petL_p2 CAACCCGCAATGAATAGGGA CGGAGGTTATAGTTAAAGCTGC 50.0 45.5 57.9 56.9 1373-1375 bp 1.0 55.3
psbE-petL_p3 CAACCCGCAATGAATAGGGA GCGGAGGTTATAGTTAAAGCTG 50.0 45.5 57.9 56.9 1374-1376 bp 1.0 55.3
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC AGCGGAGGTTATAGTTAAAGCT 50.0 40.9 57.8 57.0 1298-1300 bp 1.0 54.7
psbE-petL_p5 ACGAATAACCAACCCGCAAT AGCGGAGGTTATAGTTAAAGCT 45.0 40.9 58.2 57.0 1384-1386 bp 1.0 54.6