Hotspot

trnF-GAA-ndhJ

LSC · 48573-49168 · 596 bp

Back to Krascheninnikovia

Pi 0.00168

MarkerSeek score 59.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 596
variable_sites: 1
pi: 0.00168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCTTGAAACGGATT	55.0	45.0	58.0	58.2	769-770 bp	1.0	78.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCTTGAAACGGATT	50.0	45.0	58.1	58.2	733-734 bp	1.0	78.4
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCTTGAAACGGATT	55.0	45.0	57.9	58.2	757-758 bp	1.0	77.7
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	746-747 bp	1.0	77.4
trnF-GAA-ndhJ_p5	TGAATGGTCGGGATAGCTCA	ATCCACGCTTGAAACGGATT	50.0	45.0	58.2	58.2	778-779 bp	1.0	77.1