Hotspot

trnT-UGU-trnL-UAA

LSC · 46587-47372 · 786 bp

Back to Krascheninnikovia

Pi 0.00128
MarkerSeek score 60.10
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
786
variable_sites
1
pi
0.00128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 CAAATGCGAGGCTCTAACCT AGCCAATTAAGTCCGTAGCG 50.0 50.0 58.0 58.1 860-865 bp 1.0 79.8
trnT-UGU-trnL-UAA_p2 GCATTACAAATGCGAGGCTC AGCCAATTAAGTCCGTAGCG 50.0 50.0 57.9 58.1 866-871 bp 1.0 79.2
trnT-UGU-trnL-UAA_p3 GACTCGAACCGATGACCATC AGCCAATTAAGTCCGTAGCG 55.0 50.0 58.2 58.1 886-891 bp 1.0 78.8
trnT-UGU-trnL-UAA_p4 CAAATGCGAGGCTCTAACCT CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 848-853 bp 1.0 78.3
trnT-UGU-trnL-UAA_p5 ACAAATGCGAGGCTCTAACC AGCCAATTAAGTCCGTAGCG 50.0 50.0 58.3 58.1 861-866 bp 1.0 78.0