Hotspot

atpH-atpI

LSC · 13946-14722 · 777 bp

Back to Krascheninnikovia

Pi 0.00129
MarkerSeek score 59.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
777
variable_sites
1
pi
0.00129
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTGCAACTTTAGCCGCAG 45.0 45.0 58.0 57.9 864 bp 1.0 78.7
atpH-atpI_p2 GCCAACTCCAGGTCCAATAG TTTTGCAACTTTAGCCGCAG 55.0 45.0 58.0 57.9 913 bp 1.0 78.7
atpH-atpI_p3 AGCGGCAGAAATCAATGGAT TTTTGCAACTTTAGCCGCAG 45.0 45.0 57.9 57.9 856 bp 1.0 78.5
atpH-atpI_p4 AGCCAATCCAGCAGCAATAA TTTTGCAACTTTAGCCGCAG 45.0 45.0 57.9 57.9 880 bp 1.0 77.8
atpH-atpI_p5 AAGCGGCAGAAATCAATGGA TTTTGCAACTTTAGCCGCAG 45.0 45.0 58.2 57.9 857 bp 1.0 77.7