Hotspot

rps16-trnQ-UUG

LSC · 6599-7207 · 609 bp

Back to Krascheninnikovia

Pi 0.00493

MarkerSeek score 61.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 609
variable_sites: 3
pi: 0.00493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GGAACCTGTATGCGGTTGAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	679-680 bp	1.0	79.4
rps16-trnQ-UUG_p2	GGAACCTGTATGCGGTTGAT	TAAGGCATCGGGTTTTGGTC	50.0	50.0	58.0	58.2	709-710 bp	1.0	78.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	745-746 bp	1.0	78.6
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	775-776 bp	1.0	77.8
rps16-trnQ-UUG_p5	TTTGGAACCTGTATGCGGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.7	58.1	682-683 bp	1.0	77.5