Hotspot

trnK-UUU-rps16

LSC · 4714-5397 · 684 bp

Back to Krascheninnikovia

Pi 0.00442
MarkerSeek score 62.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
684
variable_sites
3
pi
0.00442
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTAGCCGCACTTAAAAGCC GGTGCTCAACCTACAGAAACT 50.0 47.6 58.3 57.9 783-789 bp 1.0 69.8
trnK-UUU-rps16_p2 TCTAGCCGCACTTAAAAGCC TTAAAAAGGGCGGACGTTTT 50.0 40.0 58.3 56.5 750-756 bp 1.0 66.5
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC TTTAAAAAGGGCGGACGTTT 50.0 40.0 58.3 56.5 751-757 bp 1.0 66.5
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC TTTTAAAAAGGGCGGACGTT 50.0 40.0 58.3 56.5 752-758 bp 1.0 66.5
trnK-UUU-rps16_p5 TCTAGCCGCACTTAAAAGCC GGTGCTCAACCTACAGAAAC 50.0 50.0 58.3 56.4 783-789 bp 1.0 65.7