Hotspot

petD-rpoA

LSC · 92602-92839 · 238 bp

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Pi 0.0672
MarkerSeek score 73.10
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
238
variable_sites
16
pi
0.0672
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 TGGGTATTGGCGCAACATTA TGCAAATTGAAGATCTTCGCA 45.0 38.1 57.9 56.8 365 bp 1.0 64.9
petD-rpoA_p2 GGGTATTGGCGCAACATTAC TGCAAATTGAAGATCTTCGCA 50.0 38.1 57.5 56.8 364 bp 1.0 62.1
petD-rpoA_p3 GGTATTGGCGCAACATTACC TGCAAATTGAAGATCTTCGCA 50.0 38.1 57.5 56.8 363 bp 1.0 62.1
petD-rpoA_p4 TGGGTATTGGCGCAACATTA ATGCAAATTGAAGATCTTCGCA 45.0 36.4 57.9 57.3 366 bp 1.0 60.8
petD-rpoA_p5 TGGGTATTGGCGCAACATTA TGCAAATTGAAGATCTTCGCAT 45.0 36.4 57.9 57.3 365 bp 1.0 60.8