Hotspot

rpl33-rps18

LSC · 81640-82537 · 898 bp

Back to Krameria

Pi 0.0215

MarkerSeek score 72.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 898
variable_sites: 19
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAATCG	AATCAATTCGATCCCCCGAC	50.0	50.0	56.8	57.7	1073-1082 bp	1.0	71.0
rpl33-rps18_p2	CGACACAATACGCCTAATCGA	AATCAATTCGATCCCCCGAC	47.6	50.0	58.3	57.7	1073-1082 bp	1.0	70.2
rpl33-rps18_p3	TCGACACAATACGCCTAATCG	AATCAATTCGATCCCCCGAC	47.6	50.0	58.3	57.7	1074-1083 bp	1.0	70.2
rpl33-rps18_p4	CGACACAATACGCCTAATCG	ATCAATTCGATCCCCCGACT	50.0	50.0	56.8	58.6	1072-1081 bp	1.0	68.4
rpl33-rps18_p5	CGACACAATACGCCTAATCGA	ATCAATTCGATCCCCCGACT	47.6	50.0	58.3	58.6	1072-1081 bp	1.0	67.6