Hotspot

psaJ-rpl33

LSC · 80103-81438 · 1336 bp

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Pi 0.0228

MarkerSeek score 73.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 1336
variable_sites: 30
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCTCTGTGGCACCGGTATTA	TCTGGAAATGCCCGTTGATT	50.0	45.0	58.1	57.8	1530-1545 bp	1.0	79.9
psaJ-rpl33_p2	CATATCTCTCTGTGGCACCG	TCTGGAAATGCCCGTTGATT	55.0	45.0	57.9	57.8	1536-1551 bp	1.0	79.9
psaJ-rpl33_p3	TCTCTGTGGCACCGGTATTA	ACAGCCGGTACATTCCAAAA	50.0	45.0	58.1	57.7	1488-1503 bp	1.0	79.4
psaJ-rpl33_p4	CATATCTCTCTGTGGCACCG	ACAGCCGGTACATTCCAAAA	55.0	45.0	57.9	57.7	1494-1509 bp	1.0	79.4
psaJ-rpl33_p5	TCTCTGTGGCACCGGTATTA	ATCTGGAAATGCCCGTTGAT	50.0	45.0	58.1	57.6	1531-1546 bp	1.0	78.1