Hotspot

rps4-trnT-UGU

LSC · 57614-58030 · 417 bp

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Pi 0.0121

MarkerSeek score 62.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 5
pi: 0.0121
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AAGATCGGTTCTGGCTCTTG	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.6	57.9	573-576 bp	1.0	76.9
rps4-trnT-UGU_p2	AAGATCGGTTCTGGCTCTTG	GATGGTCATCGGTTCGACTC	50.0	55.0	57.6	58.2	542-545 bp	1.0	76.8
rps4-trnT-UGU_p3	AAGATCGGTTCTGGCTCTTG	TGTTATGTGAGCCCGCTTAG	50.0	50.0	57.6	57.7	593-596 bp	1.0	75.5
rps4-trnT-UGU_p4	AAGATCGGTTCTGGCTCTTG	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	57.6	57.5	581-584 bp	1.0	73.9
rps4-trnT-UGU_p5	AAGATCGGTTCTGGCTCTTG	CAGAGGTTAGAGCATCGCAT	50.0	50.0	57.6	57.5	571-574 bp	1.0	73.8