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psbZ-trnG-GCC

LSC · 47297-47934 · 638 bp

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Pi 0.0242

MarkerSeek score 75.10

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 15
pi: 0.0242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGAAGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	57.9	57.8	781-792 bp	1.0	81.2
psbZ-trnG-GCC_p2	TTCTCCTGAAGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.3	57.8	780-791 bp	1.0	79.6
psbZ-trnG-GCC_p3	TCCTGAAGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.7	57.8	777-788 bp	1.0	79.5
psbZ-trnG-GCC_p4	TCTCCTGAAGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.7	57.8	779-790 bp	1.0	76.0
psbZ-trnG-GCC_p5	CTCCTGAAGGTTGGTCGAGT	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	59.0	57.8	778-789 bp	1.0	73.7