Hotspot

trnT-GGU-psbD

LSC · 41599-43980 · 2382 bp

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Pi 0.0131

MarkerSeek score 68.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 2382
variable_sites: 30
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	2460-2532 bp	1.0	81.4
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	2474-2546 bp	1.0	81.3
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	2463-2535 bp	1.0	81.2
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	50.0	50.0	58.1	57.7	2477-2549 bp	1.0	81.1
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	55.0	50.0	58.1	57.7	2476-2548 bp	1.0	80.9