Hotspot

FNM16_pgt005-trnR-UCU

LSC · 16346-17075 · 730 bp

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Pi 0.0404
MarkerSeek score 82.50
Primer available yes
Label FNM16_pgt005-trnR-UCU

Feature Metadata

label_name
FNM16_pgt005-trnR-UCU
feature_type
igs
region
LSC
length_bp
730
variable_sites
28
pi
0.0404
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
FNM16_pgt005-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 804-810 bp 1.0 69.8
FNM16_pgt005-trnR-UCU_p2 ACCCTTAGCCTTCCAAGCTA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 810-816 bp 1.0 69.7
FNM16_pgt005-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 810-816 bp 1.0 69.1
FNM16_pgt005-trnR-UCU_p4 ACCCTTAGCCTTCCAAGCTA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 816-822 bp 1.0 69.0
FNM16_pgt005-trnR-UCU_p5 CTAACGATGCGGGTTCGATT AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.4 56.9 793-799 bp 1.0 66.7